Hibs midfielder Lewis Stevenson is out after suffering a broken bone in his foot but Liam Miller is available.
The Irish midfielder is serving a two-game ban in the league but will be free to play at Somerset Park.
Ayr United manager Brian Reid: "I will be promoting a number of boys from our Youth Academy to fill in gaps on the substitute's bench.
"Hibs are very much the favourites still, however a large Ayr support cheering us on will motivate us considerably."
Hibernian manager Colin Calderwood: "We're very aware why the game has been taken by the television.
"I think it's an attractive game with a fair chance of an upset - we want to spoil that type of party.
"I think it's going to be a proper test of us and we'll have to be very good - we'll have to be very close to top level to have any chance of getting anything out of the game.
"We have to be very determined to make sure we get the result that everybody else doesn't want.
"There will be a winner on the night one way or another - it would be nice to get it."
Source: BBC Sport
Source: BBC Sport